H2O2 suppresses ROS elimination and osteogenesis by inhibiting MT2 expression

Hydrogen peroxide (H 2 O 2 ) can damage the activity of osteoblasts, thus inhibiting the osteogenesis.Metallothionein (MT) is a scavenger of reactive oxygen species (ROS), which can promote the osteogenesis.Our transcriptomic results showed that H 2 O 2 decreased the mRNA expression of MT2 in osteoblasts.This study aims to explore the regulatory effect of MT2 gene on the osteogenesis under oxidative stress.In this study, we explored the effect of MT2 on ROS production in osteoblasts by using gene overexpression or silencing.Then, the role of H 2 O 2 on MT2 expression in osteoblasts was further observed.Ultimately, the effect of MT2 overexpression on osteogenic capacity under H 2 O 2 treatment was investigated.Our results showed that MT2 overexpression reduced ROS production in osteoblasts, while MT2 silencing was contrary.Moreover, MT2 levels in osteoblasts decreased under H 2 O 2 intervention.Importantly, MT2 overexpression reversed H 2 O 2 -inhibited osteoblastic number and activity.In conclusion, this study indicated that H 2 O 2 can inhibit ROS scavenging by downregulating MT2 expression, whereby inhibiting the osteogenesis.