邻苯二甲酸盐
精子
过度活跃
精子活力
化学
内分泌干扰物
内分泌学
内科学
精液
男科
生物
内分泌系统
细胞生物学
激素
解剖
医学
有机化学
作者
Xinyi Sun,Wenqiong Chen,Shiqi Weng,Tingting Pan,Xiao-nian Hu,Fang Wang,Tianxinyu Xia,Houyang Chen,Tao Luo
摘要
Di-2-ethylhexyl phthalate (DEHP), a plastic-derived, endocrine-disrupting chemical, has been shown to exhibit male reproductive toxicity. However, its effects on human mature spermatozoa are largely unknown. In this study we investigated the invitro effects of DEHP and mono-2-ethylhexyl phthalate (MEHP; the main metabolite of DEHP) on sperm function and the mechanisms involved. Human spermatozoa were exposed to phthalates invitro at the doses that cover the concentrations detected in human semen: 20nM–8 μM DEHP, 1nM–20 μM MEHP or a mixture of 20nM–8 μM DEHP and 1nM–20 μM MEHP. DEHP and MEHP, alone or in combination, had no effect on the viability, membrane integrity, motility, homeostasis of reactive oxygen species or mitochondrial activity of human spermatozoa. Interestingly, 1nM–20 μM MEHP and combinations of 20nM–8 μM DEHP and 1nM–20 μM MEHP enhanced penetration ability, hyperactivation and the spontaneous acrosome reaction of human spermatozoa, and increased intracellular free Ca2+ concentrations ([Ca2+]i) and tyrosine phosphorylation, two key signalling pathways that regulate sperm function. The findings of this study suggest that invitro exposure to MEHP metabolised from DEHP affects human sperm function by inducing increases in sperm [Ca2+]i and tyrosine phosphorylation, which adds to our understanding of the effects of DEHP on male reproduction.
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