清脆的
Cas9
基因组编辑
生物
计算生物学
引导RNA
核酸酶
基因
遗传学
作者
Miao Wu,Niandan Hu,Xiongming Du,Jia Wei
出处
期刊:Briefings in Functional Genomics
[Oxford University Press]
日期:2020-02-10
卷期号:19 (3): 229-234
被引量:2
摘要
Abstract CRISPR/Cas9, as a new genome-editing tool, offers new approaches to understand and treat diseases, which is being rapidly applied in various areas of biomedical research including sepsis field. The type II prokaryotic CRISPR/Cas system uses a single-guide RNA (sgRNA) to target the Cas9 nuclease to a specific genomic sequence, which is introduced into disease models for functional characterization and for testing of therapeutic strategies. This incredibly precise technology can be used for therapeutic research of gene-related diseases and to program any sequence in a target cell. Most importantly, the multifunctional capacity of this technology allows simultaneous editing of several genes. In this review, we focus on the basic principles, advantages and limitations of CRISPR/Cas9 and the use of the CRISPR/Cas9 system as a powerful tool in sepsis research and as a new strategy for the treatment of sepsis.
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