Modification of dough characteristics and baking quality based on whole wheat flour by enzymes and emulsifiers supplementation

The objective of the present study was to evaluate the effect of enzymes (xylanase and amylase) and emulsifiers (SSL and DATEM) on the rheological properties of dough and physicochemical, textural, microstructural, nutritional, as well as sensory attributes of whole wheat bread (WWB). Based on the results, the combination of xylanase and emulsifiers had a synergetic influence on the dough rheology and bread attributes due to the interactions among enzyme-emulsifiers and bread ingredients. Incorporating xylanase, SSL, and DATEM into the whole wheat bread formulation significantly enhanced the stability, extensibility, total antioxidant activity, and general acceptability, while phytic acid content and hardness of the final bread were decreased. Regarding SEM analysis, a more homogeneous and uniformed texture was observed in the samples containing xylanase and emulsifiers, compared to the bread without any additives. On the other hand, amylase demonstrated no strengthening effect on whole wheat bread, this could be explained by its influence on the starch decomposition. WWB prepared with a combination of enzymes and emulsifiers was given higher scores in the organoleptic parameters. In conclusion, fortification of whole wheat bread with xylanase, amylase, SSL, and DATEM can play a positive role in the quality as well as the nutritional value of WWB.