Comparison of Etest and agar dilution for testing the activity of three macrolides against Bordetella parapertussis

Agar dilution and Etest using Mueller-Hinton II agar supplemented with 5% whole defibrinated horse blood were compared for testing the activities of azithromycin, clarithromycin, and erythromycin against 34 clinical isolates of the fastidious species Bordetella parapertussis. There was good overall agreement (100.0% within +/- 1 log2 dilution step) between both methods. The Etest offers an excellent and convenient alternative to agar dilution for testing the activities of macrolides against B. parapertussis.