里贝斯
DPPH
化学
细胞凋亡
阿布茨
抗氧化剂
癌细胞
生物化学
IC50型
细胞生长
细胞
分子生物学
生物
植物
癌症
体外
遗传学
作者
Ning Jia,Youling L. Xiong,Baohua Kong,Qian Liu,Xiufang Xia
标识
DOI:10.1016/j.jff.2012.01.009
摘要
A black currant extract (BCE) was prepared and its antiproliferative activity against gastric cancer SGC-7901 cells was investigated. Strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activities and a high reducing power were confirmed with BCE. BCE inhibited the proliferation of SGC-7901 cells in a dose- and time-dependent manner, and the IC50 were 12.7, 10.2 and 9.0 mg/mL for 12, 24 and 48 h, respectively. Morphologic observations with inverted and fluorescence microscopes yielded vivid evidence of cell shrinkage, formation of cytoplasmic filaments, condensation of nuclear chromatin, and cell apoptosis in the presence of BCE. Flow cytometric analysis also showed that BCE treatment at concentrations of 10–20 mg/mL resulted in marked reductions of viable cells. The high concentration of phenolic compounds present in the BCE (12.2 mg/mL), including six prominent anthocyanins identified by HPLC–ESI-MS2, appeared to be responsible for BCE’s antiradical activity and anticancer effects. These findings of inhibition of SGC-7901 cells and induction of apoptosis suggest that black currant may contribute to the reduction in gastric cancer risk.
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