化学
色谱法
离子阱
微量分析
气体分析呼吸
分子筛
挥发性有机化合物
质谱
质谱法
气相色谱法
存水弯(水管)
分析化学(期刊)
吸附
有机化学
环境科学
环境工程
作者
M Phillips,J Greenberg
标识
DOI:10.1093/clinchem/38.1.60
摘要
Abstract We describe a method for the collection and microanalysis of the volatile organic compounds in human breath. A transportable apparatus supplies subjects with purified air and samples their alveolar breath; the volatile organic compounds are captured in an adsorptive trap containing activated carbon and molecular sieve. The sample is thermally desorbed from the trap in an automated microprocessor-controlled device, concentrated by two-stage cryofocusing, and assayed by gas chromatography with ion-trap detection. Compounds are identified by reference to a computer-based library of mass spectra with subtraction of the background components present in the inspired air. We used this device to study 10 normal subjects and determined the relative abundance of the volatile organic compounds in their alveolar breath. The breath-collecting apparatus was convenient to operate and was well tolerated by human volunteers.
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