脂滴包被蛋白
虫草素
脂滴
脂解
脂肪细胞
油红O
化学
内科学
3T3-L1
内分泌学
脂肪甘油三酯脂肪酶
脂质代谢
细胞生物学
脂肪生成
生物
生物化学
脂肪组织
医学
作者
Hongyue Xu,Bingjie Wu,Xueyan Wang,Fangxue Ma,Yan Li,Yanan An,Chao Wang,Xuefei Wang,Wenjing Luan,Shulin Li,Mingyuan Liu,Jianyi Xu,Hongjuan Wang,Xudong Tang,Lu Yu
摘要
To explore the effect of cordycepin on reducing lipid droplets in adipocytes.Rats were fed a 60% high-fat diet to construct a hyperlipidaemia animal model and then treated with cordycepin at different concentrations for 8 weeks. Adipocytes were extracted, and BODIPY staining was used to detect the size of the lipid droplets. The adipocyte membrane proteins ASC-1, PAT2 and P2RX5 were assessed to determine the transformation of white adipocytes to beige and brown adipocytes. In an in vitro study, 3T3-L1 cells were cultured, and Western blotting was used to determine the expression of the lipid droplet-related genes Fsp27, perilipin 3, perilipin 2, PPAR-γ, Rab5, Rab7, Rab11, perilipin 1, ATGL and CGI-58.We found that cordycepin could promote the transformation of white adipocytes into beige and brown adipocytes. Cordycepin also downregulated the lipid droplet-associated genes Fsp27, perilipin 3, perilipin 2, Rab5, Rab11 and perilipin 1. Moreover, cordycepin reduced the expression of protein CGI-58, which inhibits lipid droplet degradation. In addition, cordycepin significantly increased the expression of ATGL, suggesting that cordycepin might stimulate lipolysis by upregulating the expression of ATGL instead of CGI-58 and by downregulating the expression of perilipin 1.Cordycepin could blockade lipid droplet formation and promote lipid droplet degradation.
科研通智能强力驱动
Strongly Powered by AbleSci AI