脚手架
材料科学
生物医学工程
椎间盘
聚己内酯
植入
细胞外基质
组织工程
再生(生物学)
体内
环空(植物学)
静电纺丝
椎间盘切除术
解剖
医学
外科
细胞生物学
腰椎
生物
复合材料
生物技术
聚合物
作者
Maude Gluais,Johann Clouet,Marion Fusellier,Cyrille Decante,C. Moraru,Maéva Dutilleul,Joëlle Véziers,Julie Lesoeur,Dominique Dumas,Jérôme Abadie,Antoine Hamel,E. Bord,Sing Yian Chew,Jérôme Guicheux,Catherine Le Visage
出处
期刊:Biomaterials
[Elsevier]
日期:2019-06-01
卷期号:205: 81-93
被引量:67
标识
DOI:10.1016/j.biomaterials.2019.03.010
摘要
Annulus fibrosus (AF) impairment is associated with reherniation, discogenic pain, and disc degeneration after surgical partial discectomy. Due to a limited intrinsic healing capacity, defects in the AF persist over time and it is hence necessary to adopt an appropriate strategy to close and repair the damaged AF. In this study, a cell-free biodegradable scaffold made of polycaprolactone (PCL), electrospun, aligned microfibers exhibited high levels of cell colonization, alignment, and AF-like extracellular matrix deposition when evaluated in an explant culture model. The biomimetic multilayer fibrous scaffold was then assessed in an ovine model of AF impairment. After 4 weeks, no dislocation of the implants was detected, and only one sample out of six showed a partial delamination. Histological and immunohistochemical analyses revealed integration of the implant with the surrounding tissue as well as homogeneously aligned collagen fiber organization within each lamella compared to the disorganized and scarcer fibrous tissue in a randomly organized control fibrous scaffold. In conclusion, this biomimetic electrospun implant exhibited promising properties in terms of AF defect closure, with AF-like neotissue formation that fully integrated with the surrounding ovine tissue.
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