Sensitive surface-enhanced Raman spectroscopy detection of thiram based on hybrid substrate

Objective To prepare hybrid substrate and apply it to detect thiram with surface-enhanced Raman spectroscopy(SERS) which provides unique molecular vibration information.Methods The Au substrate was prepared by deposition of gold film on the silver substrate that had a rough surface.The Au substrate was treated with amination as a linker with the silver sol before the hybrid substrate was formed.With PATP as a probe molecule,the Raman intensity of PATP on the Au substrate and the hybrid substrate was compared,respectively.Results and Conclusion PATP had stronger Raman intensity on hybrid substrate than on the Au,and the detection limit was 10-9mol / L.This method can be used for quantitative detection on the hybrid substrate by SERS.