Optimization of enzymatic extraction conditions of skin collagen from cod (Gadus morrhua) by response surface method

Effects of concentrations of enzyme,treating time and temperature on rate of extraction of collagen from fish-skin were studied with re-sponse surface method.The results showed that the optimum conditions were concentration of enzyme 0.054%,treating materials under 16.32℃ for 10.43 h and under the conditions,the extraction rate was 27.53%,which was 22.3% higher than that of the highest of single factor experiment.Study with SDS-PAGE showed that the extracted collagens were highly purified and consisted of chains of α1,α2,whose molecular weight of was 113 kDa and 110 kDa,respectively.