The effect of different conditions of temperature and humidity on preservations of Auricularia auricula and mushroom were studied.The indexes of Auricularia auricula included the changes of Auricularia auricula polysaccharides,color difference and sensory indexes;the indexes of mushroom included the changes of amino acids,color difference,and sensory indexes.The result showed that preservation of Auricularia auricula in lower humidity and room temperature could effectively protect its active ingredients.Mushroom need to be kept in cold storage and the ambient humidity was enough to keep it well.