The analysis on indeterminate results of HIV antibodies from 2008 to 2012 in Hunan province

Objective To analyze the reasons and characteristics of the indeterminate results of HIV antibodies in Hunan province in recent five years and provide the evidence for improving detection strategies.Methods The relationship of the outcome of the indeterminate HIV antibodies and the band distribution of western blotting test was investigated by analyzing data of epidemiological follow-up,CD+4T cell counts and viral loads collected in HIV indeterminate subjects from 2008to 2012.Results There were 2995samples treated with confirmatory test in the last five years,among which 433samples were indeterminate.The ratio of indeterminate results of HIV antibodies increased year by year,from 4.4%(21/477)in 2008to 23.0%(182/791)in 2012.And the ratio of positive cases,especially in advanced patients,increased year by year from 10.3%(3/29)and 6.9%(2/29)in 2009to 51.4%(91/177)and 35.0%(62/177)in 2012,respectively.The band distribution in negative subjects was mainly p24or gp160,generally less than two bands.The distribution in early infectors was mainly p24and p24+gp160,and the appearance ratio of p24is 100%.More bands with weak color occurred in a more complex pattern in advanced patients,mainly p24、p31、gp120and gp160.Conclusion With the increase of HIV infectors,especially advanced patients,more attention should be paid on indeterminate results to adjust the detection strategy timely and avoid missed diagnosis.