Study on proteolysis susceptibility of Alcalase to the oxidized whey protein by a free radical-generating system

The changes of active sulfhydryls in different concentrations of H2O2 or FeCl3 were investigate,the effect of protein oxidation on proteolysis susceptibility of Alcalase in different oxidative conditions,and the changes of 12% TCA-N(Trichloroacetic acid nitrogen) solubility.The results showed that protein oxidation could significantly decrease the active sulfhydryls content(P0.05) in different oxidative environment.The oxidized whey protein was more easily hydrolyzed by Alcalase.The protein oxidation could significantly increase the degree of hydrolysis(DH) and 12% TCA-N solubility(P0.05).It revealed proteolysis susceptibility was improved by protein oxidation,making protein hydrolysis more easily.It means that whey protein became more easily digestion after oxidation.