清脆的
反式激活crRNA
核糖核酸
生物
效应器
Cas9
CRISPR干扰
DNA
计算生物学
核糖核酸酶P
RNA沉默
遗传学
细胞生物学
基因
RNA干扰
作者
Omar O. Abudayyeh,Jonathan S. Gootenberg,Silvana Konermann,Julia Joung,Ian M. Slaymaker,David Benjamin Turitz Cox,Sergey Shmakov,Kira S. Makarova,Ekaterina Semenova,Leonid Minakhin,Konstantin Severinov,Aviv Regev,Eric S. Lander,Eugene V. Koonin,Feng Zhang
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2016-08-05
卷期号:353 (6299)
被引量:1666
标识
DOI:10.1126/science.aaf5573
摘要
The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated genes (Cas) adaptive immune system defends microbes against foreign genetic elements via DNA or RNA-DNA interference. We characterize the class 2 type VI CRISPR-Cas effector C2c2 and demonstrate its RNA-guided ribonuclease function. C2c2 from the bacterium Leptotrichia shahii provides interference against RNA phage. In vitro biochemical analysis shows that C2c2 is guided by a single CRISPR RNA and can be programmed to cleave single-stranded RNA targets carrying complementary protospacers. In bacteria, C2c2 can be programmed to knock down specific mRNAs. Cleavage is mediated by catalytic residues in the two conserved Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains, mutations of which generate catalytically inactive RNA-binding proteins. These results broaden our understanding of CRISPR-Cas systems and suggest that C2c2 can be used to develop new RNA-targeting tools.
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