Effects of propofol on intestinal epithelial cells with oxidative stress injury

Objective:To study the effects of propofol on IEC in the oxidative stress injury induced by H2O2.Methods: IEC were cultured in intro,which were treated with H2O2,to simulate the IEC injured by OH from H2O2.IEC were divided into three group: control group,H2O2 group(2.5mmol/L) and propofol group(100μmol/L).The IEC viability was observed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay.The lactatc dehydrogenase(LDH) that was relased into the culture media and the malondialdehyde(MDA) content in IEC were measured.Results: Compared with the control group,LDH in the culture media and MDA content in H2O2 group were significantly elevated,and the IEC viability obviously decreased(P0.01).LDH in the culture media and MDA content in propofol group were lower than those of H2O2 group,and the IEC viability was obviously higher than that of H2O2 group.Conclusion: Propofol can protect rat IEC from the oxidative stress injury.