Prokaryotic Expression and Purification of the Specific Glycoprotein Antigens of Herpes Simplex Virus

To express and purify the specific glycoprotein antigens of herpes simplex virus (HSV) as used in HSV clinical detection. HSV type-common glycoprotein gD gene and type-specific glycoproteins gG1(typeⅠ) and gG2 (typeⅡ) genes amplified by PCR were subcloned into prokaryotic vector pQE30 and expressed in E.coli M15. HSV glycoprotein gD was highly expressed and purified from the inclusion body. HSV specific glycoproteins were expressed successfully in prokaryotic system therefore provides the basis for further research on HSV clinical detection.