Determination of Matrine and Oxymatrine in Sophora flavescens Alt and Sophora viciifoli

The contents of matrine and oxymatrine in the wildness Sophora flavescens Alt and Sophora viciifoli were detected by HPLC with ZORBAX SB-C18 (150mm×4.6mm, 5μm) column at 20℃. The mobile phase consisted of 0.04 mol/L H3PO4 (pH=1.8)-CH3OH (90:10).The flow rate was 1.0 mL/min and detection wave length was at 220 nm. The results revealed that the high content of matrine in the wild Sophora flavescens Alt especially in its root was observed. The content of matrine in root reached 0.34%. On the other hand, 2.45% oxymatrine in the Sophora viciifoli seed which was higher than Sophora flavescens Alt indicated that Sophora viciifoli had a good utilization value for producing matrine.