Using different over-wintering pathogen sources of watermelon bacterial fruit blotch and different inoculated-pathogen methods to test the pathogenicity of Xinong No.9.The results showed that the over-wintering pathogen sources of diseased fruit extract,dry diseased leaf,fresh diseased leaf and bacteria carrying soil all had pathogenicity,in which the over-wintering pathogen sources of bacteria carrying soil and dry diseased leaf had stronger pathogenicity;four different inoculated-pathogen methods(seed soaking,root drenching,needle inoculation and spraying) had a certain impact on pathogenicity of different over-wintering pathogen sources,in which the needle inoculation method had the strongest pathogenicity.Therefore,the results provide a scientific base for popularizing control measures,such as deep tillage,weed out diseased residues in filed,soil disinfection,reducing wounds caused by filed operation,etc..