Pharmacokinetics of Puerarin in Pueraria and Its Compound Preparation in Rat

OBJECTIVE To develop a RP-HPLC method for the determination of the concentration of puerarin in rat plasma,to study the pharmacokinetics of pueraria extradite and Naodesheng tablet,and to investigate the effect of other components in compound on the pharmacokinetics of puerarin.METHODS The rats received 100 mg·kg-1 pueraria extract and Naodesheng tablet by oral administration.After liquide extraction with methanol,plasma concentration of puerarin was measured.The pharmacokinetic parameters were calculated with 3P97 program.RESULTS A good linear relationship of puerarin was obtained in the range of 10 and 1 000 μg·L-1,the lowest limit of determination was 6 μg·L-1.The mean recoveries were 89.9%,95.5%,89.7% for high,middle,low concentrations of the samples respectively.The plasma concentration-time curves of puerarin were fitted with two-compartments model.The AUC0→∞ and ρmax of puerarin were increased in the Naodesheng group,compared with 100 mg·kg-1 pueraria extract group.CONCLUSION The HPLC method was selective,accurate and sensitive.The results indicated that the other herbs improved the absorption of puerarin and increased the bioavailability of puerarin significantly.