Review of diagnosis methods and vaccine research of enterovirus 71

The diagnosis method and vaccine research were summarized.There are several diagnosis methods for enterovirus 71(EV71),such as separation and culture of virus,titer test of neutralizing antibody,nucleic acid detection and immunological assay.The main research contents of EV71 vaccine are attenuated live vaccine based on the C4 strain and evaluated through animal model,virus like particle vaccine and recombinant vaccine aimed at VP1 site.It is provided one effective method for rapid diagnosis of EV71 combined use of two or more diagnosis assay.The foundation of suitable animal model,the screening of virus strain with high immunogenicity and cross protection,and the comparison of immunogenicity for different vaccine are the direction of virus research.