Pingyangmycin-Regulated Expression of Vascular Cell Adhesion Molecule-1(VCAM-1),Intercellular Adhesion Molecule-1(ICAM-1) and Intercellular Adhesion Molecule-3(ICAM-3) in Human Venous Malformation Endothelial Cells

Objective: To investigate PYM-regulated expressions of VCAM-1,ICAM-1 and ICAM-3 in primary cultured HVMECs.Methods: Expressions of the adhesion molecules VCAM-1,ICAM-1 and ICAM-3 were studied in PYM-regulated HVMECs in vitro by means of ELISA and RT-PCR.Results: Expressions of VCAM-1 and ICAM-3 were induced,and expression of ICAM-1 was up-regulated,both in a time and concentration-dependent fashion after stimulation with PYM.The expression of VCAM-1 was observed at 2h and ICAM-1 at 6h and ICAM-3 at 12h.The highest expression of VCAM-1,ICAM-1 and ICAM-3 was observed at 8h,18h and 24h.After exposed for the same time interval,expression of adhesion molecules on HVMECs exposed to 1mg/L of PYM was higher than that exposed to other concentration of PYM.mRNA expressions of VCAM-1,ICAM-1 and ICAM-3 started at 2h,6h and 12h respectively.Maximal synthetic activity was observed at 6-8h for VCAM-1,at 12-18h for ICAM-1 and at 18-24h for ICAM-3.Synthesis activity was greatly suppressed at 10mg/L or higher concentration.Conclusion: Expression of Ig-like adhesion molecules in HVMECs can be induced or up-regulated by lower concentration of PYM in a time and concentration-dependent fashion.