Preparation and preliminary detection of polyclonal antibody against the causal agent of sugarcane ratoon stunting disease

In the present study,the causal bacterium of sugarcane ratoon stunting disease was isolated,purified and extensively cultured,and then using as antigen,the polyclonal antibody specific for the RSD bacterium was produced by immunizing the healthy rabbits in several times.The titer of the polyclonal antibody was detected using indirect enzyme-linked immunoadsordent assay(ELISA),and the working dilution proportion was confirmed by dot blot enzyme immunoassays(DB-EIA).In addition,the detection effect of this antibody was compared with that from Australia.The results showed that the titer of the polyclonal antibody obtained was above 1∶10000,and the best working dilution proportion was 10 folds.Moreover,the result of the detection of four samples was consistent with the RSD antibody from Australia,except that the blotting color was a little lighter.To sum up,the titer of the RSD polyclonal antibody prepared in our study was a little lower than that of the RSD polyclonal antibody from Australia,but with sensitive detection effect,and could be well used for the detection of RSD in the sugarcane industry in the future.