Research on DNA Extraction Methods from a Single Nematode

To explore an efficient,stable,low-cost DNA extraction method from single nematode for the support of molecular identification of nematodes,using six species of plant-parasitic nematodes as experimental materials, four DNA extraction methods,namely,direct lysis method,freeze-thaw lysis method,cutting lysis method,freeze-thaw cutting lysis method were designed and compared in order to check their efficiency of DNA extraction from single nematode. The results showed that cutting lysis method and freeze-thaw cutting lysis method were the most efficient and stable methods for DNA extraction of single nematode,then the freeze-thaw lysis method,and the direct lysis method was the worst one. Total DNA with high-quality from a single nematode could be gained efficiently and stably through two methods,namely,cutting lysis method and freeze-thaw cutting lysis method. And the extracted DNA meeted requirement of the following PCR amplification.