Effect of PEG Modification on Antigenicity of β-Lactoglobulin

β-Lactoglobulin(β-LG) was modified by suecinimidyl carbonate methoxy polyethlene glycol(SC-mPEG) under different conditions of molar ratio,reaction time and pH.The change of its antigenicity was also studied.Indirect competitive ELISA was used to detect the antigenicity of the SC-mPEG-β-LG mixture.The results indicated that the highest reduction rate of antigenicity for modified products under different conditions was 70.2% at the reaction time of 8 h and at the same time,the highest modification degree detected by TNBS was 39.1%.Meanwhile,reaction time was the major factor affecting antigenicity reduction of β-LG modified by SC-mPEG.