Methods for total high-quality protein extraction from banana fruit.

Three methods of total protein extraction from banana(Musa AAA Group Cavendish)fruit,including trichloroacetic acid/acetone precipitation method,phenol extraction method and acetone precipitation method were compared in this paper.The results showed that the spectrum of proteins extracted with phenol extraction method was complete and clear;while that obtained with trichloroacetic/acetone precipitation was blurred and smearing,and using acetone precipitation resulted in low protein recovery and the protein bands were fewer,so it showed that the phenol extraction was suitable for extracting total protein from banana fruit.With improved extraction buffer solution,this method achieved a good result when used to extract proteins from banana fruit treated with different hormones.This study provides a useful method for banana fruit protein research.