Electrochemical determination of 8-hydroxydeoxyguanosine using a carbon nanotube modified electrode

In this work, a multi-wall carbon nanotube (MWNT) film-modified glassy carbon electrode (GCE) was constructed for the determination of 8-hydroxydesoxyguanosine (8-OHdG). The electrochemical behaviors of 8-OHdG were examined using cyclic voltammetry (CV) and linear sweep voltammetry (LSV), suggesting that MWNT film facilitates the electron transfer of 8-OHdG and then significantly enhances the oxidation peak current of 8-OHdG. Finally, a sensitive and simple electrochemical method with a good linear relationship in the range of 8.0 × 10 −8 ∼ 5.0 × 10 −6 mol 1 −1 , was developed for the determination of 8-OHdG. The detection limit is 9.0 × 10 −9 mol 1 −1 for 6-min accumulation. This newly-proposed method was successfully used to detect 8-OHdG in urine samples.