肌内脂肪
多不饱和脂肪酸
脂肪生成
脂蛋白脂酶
内分泌学
内科学
生物
脂肪组织
脂多糖学
脂肪酸
脂肪细胞
化学
动物科学
食品科学
临床化学
生物化学
医学
作者
Huimin Luo,Hong Wei,Feiruo Huang,Zheng Zhou,Siwen Jiang,Jian Peng
出处
期刊:Lipids
[Wiley]
日期:2009-10-02
卷期号:44 (11)
被引量:40
标识
DOI:10.1007/s11745-009-3346-y
摘要
Abstract The aim of the study was to investigate the effect of n‐3 PUFA enrichment in longissimus muscle on intramuscular fat (IMF) content and expression of related genes in growing‐finishing barrows. Two isoenergetic, isonitrogenous and isolipidic diets were formulated: one was basal diet and the other contained 10% linseed. Twenty‐four Landrace × NewDamLine barrows weighing 35 ± 3.7 kg were randomly assigned to four treatment groups with six pigs per group. During the whole experimental period of 90 days, all groups were first fed the basal diet and then the linseed diet for 0, 30, 60, and 90 days before slaughter, respectively. Meat quality, fatty acid composition, and expression of genes involved in adipogenesis in longissimus muscle were measured and analyzed. The IMF content increased linearly ( P < 0.05) as the linseed diet feeding time prolonged. Meanwhile, n‐3 PUFA content and expression of peroxisome proliferator‐activated receptor δ ( PPARδ ), PPARγ , adipocyte fatty acid–binding protein ( aP2 ) and lipoprotein lipase ( LPL ) increased linearly ( P < 0.01) as well, while the expression of wingless related MMTV integration site 10b ( Wnt10b ) linearly decreased ( P < 0.01). Furthermore, significant ( P < 0.01) quadratic or linear relation was observed between n‐3 PUFA enrichment and expression of these genes, while significant ( P < 0.01) quadratic or linear relation was observed between the expression of PPARγ , aP2 or Wnt10b and IMF content. These data show that enhancing n‐3 PUFA enrichment in muscle leads to significant increase in IMF content. A possible explanation is due to alterations in the expression of genes involved in adipogenesis, however this will need to be confirmed by protein and enzyme activity studies.
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