核糖核酸酶P
核糖核酸
劈理(地质)
劈开
核糖核酸酶H
核糖核酸酶Ⅲ
转录后修饰
生物化学
核糖核酸酶
化学
生物
分子生物学
非编码RNA
酶
基因
RNA干扰
古生物学
断裂(地质)
作者
Anthony Forster,Sidney Altman
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1990-08-17
卷期号:249 (4970): 783-786
被引量:287
标识
DOI:10.1126/science.1697102
摘要
Ribonuclease P (RNase P) from Escherichia coli or its catalytic RNA subunit can efficiently cleave small RNA substrates that lack the conserved features of natural substrates of RNase P if an additional small RNA is also present. This additional RNA must contain a sequence complementary to the substrate [external guide sequence (EGS)] and a 3'-proximal CCA sequence to ensure cleavage. The aminoacyl acceptor stem and some additional 5'- and 3'-terminal sequences of a precursor transfer RNA are sufficient to allow efficient cleavage by RNAase P, and the 2'-hydroxyl group at the cleavage site is not absolutely necessary for cleavage. In principle, any RNA could be targeted by a custom-designed EGS RNA for specific cleavage by RNase P in vitro or in vivo.
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