生物
DNA复制
分子生物学
DNA聚合酶
复制因子C
DNA聚合酶Ⅱ
染色体复制控制
DNA钳
真核细胞DNA复制
DNA
原点识别复合体
聚合酶
遗传学
逆转录酶
基因
核糖核酸
作者
Bruce Stillman,Fuyuhiko Tamanoi,Michael B. Mathews
出处
期刊:Cell
[Elsevier]
日期:1982-12-01
卷期号:31 (3): 613-623
被引量:140
标识
DOI:10.1016/0092-8674(82)90317-8
摘要
Temperature-sensitive mutants in the N complementation group of human adenovirus type 5 are defective at the nonpermissive temperature for replication of virus DNA and for transformation of rat embryo cells. We show that nuclear extracts prepared from Ad5ts149-infected cells grown at the nonpermissive temperature fail to replicate DNA in vitro. The defect lies in the first step in the initiation of viral DNA synthesis, the formation of a covalent linkage between the terminal protein precursor (pTP) and dCMP. A 140 kilodalton (140 kd) protein which complements these defective extracts and contains DNA polymerase activity has been purified from HeLa cells infected with wild-type Ad2. It is tightly associated with the 80 kd pTP in a replication complex. Both of these proteins are products of the E2B region of the adenovirus genome, and the 140 kd protein coding sequences lie immediately downstream from those encoding the 80 kd protein. These results demonstrate that adenovirus encodes a novel DNA polymerase that is required for priming of DNA synthesis at the origin of replication. This protein may also function in the initiation of transformation of cultured cells.
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