卵黄原蛋白
生物
卵黄发生
细胞质
蛋黄
分泌物
吖啶橙
内科学
内分泌学
卵黄蛋白原
细胞生物学
生物化学
卵母细胞
生态学
基因
胚胎
细胞凋亡
医学
标识
DOI:10.1016/0022-1910(86)90088-0
摘要
Trophocytes of the mosquito fat body produce large amounts of the yolk-protein precursor, vitellogenin, that is then accumulated by developing oöcytes. Vitellogenin synthesis is a highly regulated process and is turned on and off according to the stage of the reproductive cycle; it is maximal 27 h after blood feeding and then rapidly declines. Here, I report a new mechanism involved in cellular regulation of vitellogenin secretion, e.g. the lysosomal breakdown of vitellogenin. The activities of four lysosomal enzymes rose sharply about 30 h after blood-feeding and remained high for the next 12 h. Video-enhanced fluorescence, combined with visualization of vitellogenin by immunolabelling and of lysosomes by acridine orange, demonstrated that this intense lysosomal activity was directed toward the specific degradation of vitellogenin; the latter diminished in the cytoplasm and was concentrated in large lysosomes, only later did it disappear entirely from the trophocytes.
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