类黄酮
葡萄糖基转移酶
糖基转移酶
苷元
葡萄糖基转移酶
槲皮素
化学
生物化学
蔗糖
葡萄糖苷
酶
糖
查尔酮合酶
糖苷
立体化学
抗氧化剂
替代医学
病理
医学
出处
期刊:Journal of Microbiology and Biotechnology
[Journal of Microbiology and Biotechnology]
日期:2009-01-01
被引量:5
摘要
Enzymatic glucosylation with glycosyltransferases can be used to regulate the water solubility of aglycone. The drawback of this process is the demand of UDP-glucose as a sugar donor. We made an in-frame fusion of the flavonoid O-glucosyltransferase (OsUGT-3) and sucrose synthase (AtSUS) genes. The resulting fusion protein, OsUGT3-AtSUS, was expressed in E. coli and purified. When sucrose and UDP were supplied, SUS-UGT was able to convert quercetin into quercetin O-glucoside without the addition of UDP-glucose. In addition, UDPglucose was recycled when sucrose was added to the reaction mixture. This fusion protein is useful for the enzymatic production of flavonoid O-glucosides.
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