Effects of Aquatic Acidification on Microbially Mediated Nitrogen Removal in Estuarine and Coastal Environments

Acidification of estuarine and coastal waters is anticipated to influence nitrogen (N) removal processes, which are critical pathways for eliminating excess N from these ecosystems. We found that denitrification rates decreased significantly under acidified conditions (P < 0.05), which reduced by 41-53% in estuarine and coastal sediments under an approximately 0.3 pH reduction of the overlying water. However, the N removal rates through the anaerobic ammonium oxidation (anammox) process were concomitantly promoted under the same acidification conditions (increased by 47-109%, P < 0.05), whereas the total rates of N loss were significantly inhibited by aquatic acidification (P < 0.05), as denitrification remained the dominant N removal pathway. More importantly, the emission of nitrous oxide (N2O) from estuarine and coastal sediments was greatly stimulated by aquatic acidification (P < 0.05). Molecular analyses further demonstrated that aquatic acidification also altered the functional microbial communities in estuarine and coastal sediments; and the abundance of denitrifiers was significantly reduced (P < 0.05), while the abundance of anammox bacteria remained relatively stable. Collectively, this study reveals the effects of acidification on N removal processes and the underlying mechanisms and suggests that the intensifying acidification in estuarine and coastal waters might reduce the N removal function of these ecosystems, exacerbate eutrophication, and accelerate global climate change.