生物
九氟化硫
家蚕
病毒学
抗体
寄生虫寄主
分子生物学
融合蛋白
重组DNA
黑腹果蝇
病菌
家蚕
微生物学
遗传学
基因
夜蛾
万维网
计算机科学
作者
Renze Zhang,Shi-Yi Zheng,Hongyun Huang,Xi Sun,Yukang Huang,Junhong Wei,Guoqing Pan,Chun-Feng Li,Zeyang Zhou
出处
期刊:Bulletin of Entomological Research
[Cambridge University Press]
日期:2022-04-06
卷期号:112 (4): 502-508
被引量:1
标识
DOI:10.1017/s0007485321001036
摘要
Nosema bombycis is a destructive and specific intracellular parasite of silkworm, which is extremely harmful to the silkworm industry. N. bombycis is considered as a quarantine pathogen of sericulture because of its long incubation period and horizontal and vertical transmission. Herein, two single-chain antibodies targeting N. bombycis hexokinase (NbHK) were cloned and expressed in fusion with the N-terminal of Slmb (a Drosophila melanogaster FBP), which contains the F-box domain. Western blotting demonstrated that Sf9-III cells expressed NSlmb-scFv-7A and NSlmb-scFv-6H, which recognized native NbHK. Subsequently, the NbHK was degraded by host ubiquitination system. When challenged with N. bombycis, the transfected Sf9-III cells exhibited better resistance relative to the controls, demonstrating that NbHK is a prospective target for parasite controls and this approach represents a potential solution for constructing N. bombycis-resistant Bombyx mori.
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