品脱1
诱导多能干细胞
清脆的
生物
泛素连接酶
细胞生物学
胚胎干细胞
遗传学
分子生物学
泛素
粒体自噬
自噬
基因
细胞凋亡
作者
Carol X.-Q. Chen,Zhipeng You,Narges Abdian,Julien Sirois,Irina Shlaifer,Mahdieh Tabatabaei,Marie-Noëlle Boivin,Lydiane Gaborieau,Jason Karamchandani,Lenore K. Beitel,Edward A. Fon,Thomas M. Durcan
标识
DOI:10.1101/2022.02.25.482014
摘要
Autosomal recessive mutations in either PRKN or PINK1 are associated with early-onset Parkinson's disease. The corresponding proteins, PRKN, an E3 ubiquitin ligase, and the mitochondrial serine/threonine-protein kinase PINK1 play a role in mitochondrial quality control. Using CRISPR/CAS9 technology we generated three human iPSC lines from the well characterized AIW002-02 control line. These isogenic iPSCs contain homozygous knockouts of PRKN (PRKN-KO, CBIGi001-A-1), PINK1 (PINK1-KO, CBIGi001-A-2) or both PINK1 and PRKN (PINK1-KO/PRKN-KO, CBIGi001-A-3). The knockout lines display normal karyotypes, express pluripotency markers and upon differentiation into relevant brain cells or midbrain organoids may be valuable tools to model Parkinson's disease.
科研通智能强力驱动
Strongly Powered by AbleSci AI