免疫系统
生物
CD14型
尿路上皮细胞
CD8型
转录组
免疫学
尿路上皮
细胞生物学
泌尿系统
基因
基因表达
遗传学
内分泌学
作者
Peng Liao,Xi Jin,Boya Li,Xiao Zeng,Banghua Liao,Tao Jin,Jiawei Chen,Xiaoshuai Gao,Wei Wang,Quanguo He,Guo Chen,Ling Gong,Hong Shen,Kun-Jie Wang,Hong Li,Deyi Luo
标识
DOI:10.1038/s41392-022-00962-8
摘要
Interstitial cystitis (IC) is a severely debilitating and chronic disorder with unclear etiology and pathophysiology, which makes the diagnosis difficult and treatment challenging. To investigate the role of immunity in IC bladders, we sequenced 135,091 CD45+ immune cells from 15 female patients with IC and 9 controls with stress urinary incontinence using single-cell RNA sequencing (scRNA-seq). 22 immune subpopulations were identified in the constructed landscape. Among them, M2-like macrophages, inflammatory CD14+ macrophages, and conventional dendritic cells had the most communications with other immune cells. Then, a significant increase of central memory CD4+ T cells, regulatory T cells, GZMK+CD8+ T cells, activated B cells, un-switched memory B cells, and neutrophils, and a significant decrease of CD8+ effector T cells, Th17 cells, follicular helper T cells, switched memory B cells, transitional B cells, and macrophages were noted in IC bladders. The enrichment analysis identified a virus-related response during the dynamic change of cell proportion, furthermore, the human polyomavirus-2 was detected with a positive rate of 95% in urine of patients with IC. By integrating the results of scRNA-seq with spatial transcriptomics, we found nearly all immune subpopulations were enriched in the urothelial region or located close to fibroblasts in IC bladders, but they were discovered around urothelium and smooth muscle cells in control bladders. These findings depict the immune landscape for IC and might provide valuable insights into the pathophysiology of IC.
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