化学
色谱法
长春瑞滨
选择性反应监测
三级四极质谱仪
液相色谱-质谱法
生物分析
洗脱
串联质谱法
长春碱
质谱法
分析物
医学
外科
化疗
顺铂
作者
Lisa T van der Heijden,A. Gebretensae,Bram Thijssen,L. van Andel,A. Laura Nijstad,Y. Wang,Hilde Rosing,Alwin D. R. Huitema,Jos H. Beijnen
标识
DOI:10.1016/j.jpba.2022.114772
摘要
A highly sensitive method was developed for the quantification of vinblastine, vincristine, vinorelbine, and its active metabolite 4-O-deacetylvinorelbine in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Deuterated isotopes were used as internal standard and liquid-liquid extraction with tert-butyl methyl ether (TBME) was used for sample pre-treatment. The final extract was injected on a C18 column (50 × 2.1 mm ID, 5 µm). Gradient elution was used in combination with Reversed Phase chromatography to elute the analytes and internal standards from the column in 5 min and the API4000 triple quadrupole MS detector was operating in the positive ion mode. The calibration model, accuracy and precision, selectivity and specificity, dilution integrity, carryover, matrix factor and recovery, and stability were evaluated over a concentration range from 0.025 to 10 ng/mL for vinblastine, vinorelbine, and 4-O-deacetylvinorelbine and from 0.1 to 40 ng/mL for vincristine. The intra- and inter-assay bias and precisions were within ± 12.4% and ≤ 10.6%, respectively. This method was successfully applied to study the pharmacokinetics of vincristine in paediatrics and vinorelbine and 4-O-deacetylvinorelbine using in vivo mouse models.
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