条形码
纳米孔
编码
核酸
计算生物学
纳米孔测序
解码方法
DNA
生物
计算机科学
DNA测序
纳米技术
基因
遗传学
材料科学
操作系统
电信
作者
Shuanghong Yan,Liying Wang,Yuqin Wang,Zhenyuan Cao,Shanyu Zhang,Xiaoyu Du,Pingping Fan,Panke Zhang,Hong‐Yuan Chen,Shuo Huang
标识
DOI:10.1002/anie.202116482
摘要
A large collection of unique molecular barcodes is useful in the simultaneous sensing or screening of molecular analytes. Though the sequence of DNA has been widely applied to encode for molecular barcodes, decoding of these barcodes is normally assisted by sequencing. We here demonstrate a barcode system based solely on self-assembly of synthetic nucleic acids and direct nanopore decoding. Each molecular barcode is composed of "n" distinct information nodes in a non-binary manner and can be sequentially scanned and decoded by a Mycobacterium smegmatis porin A (MspA) nanopore. Nanopore events containing step-shaped features were consistently reported. 14 unique information nodes were developed which in principle could encode for 14n unique molecular barcodes in a barcode containing "n" information nodes. These barcode probes were adapted to detect different antibody proteins or cancer-related microRNAs, suggesting their immediate application in a wide variety of sensing applications.
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