Global proteomic analysis of acetaminophen toxicity in 3D human liver microtissues

Introduction Early prediction of drug safety remains a challenge in preclinical phases of drug development. Global proteomic analysis allows for a better understanding of the mode of action and mechanism of toxicity induced by drug treatment. We applied a novel mass-spectrometric approach Hyper Reaction Monitoring (HRM) – to quantify changes in protein expression in 3D InSightTM Human Liver Microtissues exposed to acetaminophen.