The Distribution of Neuronal and Inducible Nitric Oxide Synthase in Urethral Stricture Formation

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 May 2004The Distribution of Neuronal and Inducible Nitric Oxide Synthase in Urethral Stricture Formation ANDRE G. CAVALCANTI, SELCUK YUCEL, DONNA Y. DENG, JACK W. McANINCH, and LAURENCE S. BASKIN ANDRE G. CAVALCANTIANDRE G. CAVALCANTI More articles by this author , SELCUK YUCELSELCUK YUCEL More articles by this author , DONNA Y. DENGDONNA Y. DENG More articles by this author , JACK W. McANINCHJACK W. McANINCH More articles by this author , and LAURENCE S. BASKINLAURENCE S. BASKIN More articles by this author View All Author Informationhttps://doi.org/10.1097/01.ju.0000121261.03616.63AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: The distribution of neuronal (n) and inducible (i) nitric oxide synthase (NOS) may have a role in the maintenance of normal urethral spongiosum and during the development of spongiofibrosis in urethral stricture disease. Materials and Methods: Eight normal and 33 strictured human bulbar urethras were studied by histological and immunohistochemical techniques for the neuronal markers S-100, nNOS and iNOS. The smooth muscle-to-collagen ratio was calculated by morphometric analysis of Masson’s trichrome sections. Immunohistochemical staining patterns of the neuronal markers in normal urethral tissue was compared to that in urethral stricture tissue with spongiofibrosis. Results: The smooth muscle-to-collagen ratio was significantly lower in the strictured urethra compared to that in the control group (p = 0.001). In the strictured bulbar urethra nNOS immunoreactivity was decreased compared to that in normal urethral tissue. The severity of spongiofibrosis corresponded to the loss of nNOS immunoreactivity. iNOS immunoreactivity was found in strictured urethral epithelium and spongiosal tissue, whereas the control group was nonimmunoreactive to iNOS. Conclusions: Urethral stricture formation is a fibrotic process associated with significant changes in NOS metabolism. Abnormal collagen synthesis following urethral trauma may be stimulated by inappropriate iNOS activity. A functional nerve supply to the urethral spongiosum seems to be crucial in the maintenance of the unique ultrastructure of the urethral spongiosum. References 1 : Biochemical characterization and quantitation of the collagenous components of urethral stricture tissue. 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Link, Google Scholar From the Department of Urology and Pediatrics, University of California-San Francisco Children’s Medical Center, University of California-San Francisco, San Francisco, California© 2004 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byBreyer B, McAninch J, Whitson J, Eisenberg M, Mehdizadeh J, Myers J and Voelzke B (2009) Multivariate Analysis of Risk Factors for Long-Term Urethroplasty OutcomeJournal of Urology, VOL. 183, NO. 2, (613-617), Online publication date: 1-Feb-2010.Mundy A (2018) RE: EDITORIAL COMMENT ON INTERIM OUTCOMES OF DORSAL SKIN GRAFT BULBAR URETHROPLASTYJournal of Urology, VOL. 174, NO. 1, (397-398), Online publication date: 1-Jul-2005. Volume 171Issue 5May 2004Page: 1943-1947 Advertisement Copyright & Permissions© 2004 by American Urological Association, Inc.Keywordsneural constitutive nitric oxide synthasefibrosisinducible nitric oxide synthaseurethral strictureurethraMetricsAuthor Information ANDRE G. CAVALCANTI More articles by this author SELCUK YUCEL More articles by this author DONNA Y. DENG More articles by this author JACK W. McANINCH More articles by this author LAURENCE S. BASKIN More articles by this author Expand All Advertisement PDF downloadLoading ...