The potential toxicity in riverbed sediments was assessed with a bioassay using the bioluminescent bacteria Vibrio fischeri . The selected area was characterized by the presence of ultramafic rocks (peridotites), and the sediments had high values in Ni, Cr, and Co. For the toxicity bioassay with Vibrio fischeri , water-soluble forms were used. The results indicated that most of the samples had a very low degree of toxicity, with 10% of reduction in luminescence in relation to the control; meanwhile 25% of the samples had a moderate degree of toxicity with a reduction in luminescence between 13 and 21% in relation to the control. The toxicity index correlated significantly with the concentrations of Ni and Cr in the water extracts. This toxicity bioassay was proved to be a sensitive and useful tool to detect potential toxicity in solutions, even with anomalous concentrations in heavy metals of natural origin.