Glypican 3型
癌症研究
癌变
转移
肝细胞癌
细胞周期
细胞生长
小发夹RNA
细胞
转染
生物
病理
细胞凋亡
癌症
医学
基因敲除
细胞培养
内科学
生物化学
遗传学
作者
Zhizhen Dong,Min Yao,Li Wang,Junling Yang,Dengfu Yao
出处
期刊:Mini-reviews in Medicinal Chemistry
[Bentham Science]
日期:2015-02-16
卷期号:14 (14): 1183-1193
被引量:9
标识
DOI:10.2174/1389557515666150101105135
摘要
Hepatocellular carcinoma (HCC) prognosis is very poor, its early treatment is of the utmost importance. Glypican-3 (GPC-3), a membrane-associated heparan sulfate proteoglycan, plays a crucial role in cell proliferation and metastasis, particularly in progression. GPC-3 mediated oncogenesis involves signaling pathways during the malignant transformation of hepatocyte carcinogenesis, with an increasing expression of GPC-3 observed from non-cancerous- to cancerous-tissues, with brown granule-like staining localized in tumor parts of atypical hyperplasia and HCC formation. GPC-3 expression in HCC tissues or circulating blood was associated with tumor size or HBV infection. Circulation of GPC-3-mRNA was detected in HCC patients with relation to TNM stage, periportal cancerous embolus, and extra-hepatic metastasis. After hepatoma, cells were transfected with shRNA, GPC-3 expression or proliferation was inhibited with promoting apoptosis, cell cycle arrested in G1 phase, alteration of hepatoma cell migration and invasion behaviors with down-regulation of β-catenin, IGF-II, and VEGF, and growth of nude mice xenograft tumors was significantly suppressed with the decreases in β-catenin, p-GSK3β, and cyclinD1 expression, suggesting that GPC-3 not only is a specific biomarker for HCC diagnosis, but also is a valuable molecular-target for HCC gene therapy.
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