Molecular Determinants for Subcellular Localization of PSD-95 with an

Summary only for clustering but also for subcellular localization of membrane proteins. The MAGUK protein Discs-large, Ion channels and PSD-95 are colocalized in specific for instance, is necessary for correct localization of Fas-neuronal subcellular locations by an unknown mecha- ciclin II and Shaker to the neuromuscular junction (Teje- nism. To investigate mechanisms of localization, we dor et al., 1997; Thomas et al., 1997; Zito et al., 1997). usedbiolistictechniquestoexpressGFP-taggedPSD- In addition, disrupting the PDZ-containing protein INAD 95 (PSD-95:GFP) and the K 1 -selective channel Kv1.4 prevents the TRP channel from localizing to the rhab- in slices of rat cortex. In pyramidal cells, PSD-95:GFP domere of the photoreceptor (Shieh and Zhu, 1996; required a single PDZ domain and a region including Chevesich et al., 1997; Tsunoda et al., 1997). In Caeno- the SH3 domain for localization to postsynaptic sites. rhabditis elegans , mutating the PDZ domain-containing When transfected alone, PSD-95:GFP was present in