骨组织
双光子激发显微术
骨髓
间充质干细胞
病理
多光子荧光显微镜
活体显微镜检查
生物医学工程
体内
医学
荧光显微镜
生物
荧光
光学
物理
生物技术
作者
Hiroki Mizuno,Masaru Ishii
出处
期刊:PubMed
日期:2018-01-27
卷期号:28 (2): 181-185
摘要
Bone tissue consist of a wide variety of cells such as osteoclasts, osteoblasts and osteocytes which are involved in bone metabolism, hematopoietic cells which can differentiate and mature in the bone marrow, other mesenchymal cells and nerve cells. Recent advances in "fluorescent imaging technology" have made it possible to observe bone tissue alive. And intravital imaging enable us not only to examine the "morphology" but also to analyze the "dynamics" of the cells. We have improved "two-photon microscope" which can observe deep tissue with minimally invasive manner and have established an imaging method to observe the movement of cells in living bone tissue in real time. In this review, we summarize the methodology of intravital imaging, such as the principle of two-photon excitation microscope, method of in vivo imaging of bone, and analysis of acquired imaging data.
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