Integrated management of rhizoctonia root rot disease of soybean caused by Rhizoctonia solani

Integration of bio-agent, fungicide and organic amendment was applied for the management of rhizoctonia root rot disease of soybean caused by Rhizoctonia solani.Before going to the field experiment, in vitro trials were conducted to select a virulent isolate of R. solani, an effective antagonistic isolate of Trichoderma harzianum, suitable fungicide and organic amendment.Among the ten isolates of R. solani, RS-5 isolate was selected as a test pathogen by the pathogenicity test.On the other hand, among the eighty-seven isolates of T. harzianum, ISR-26 isolate depicted the highest (77.37%) inhibition of radial growth of test pathogen.In the case of fungicidal evaluation trial, Conza 5% EC, Bavistin 50 WP and Provax 200 WP were found the most effective fungicide at all concentration such as 75, 150 and 300 ppm.Additionally, in vitro trial of different organic amendments, mustard oil cake was found the most active organic amendment for reducing the radial growth (66.29%) and mycelial dry weight (73.46%) at the level of 3% concentration.In the field trial, integrated use of T. harzianum with Provax 200 WP and mustard oil cake under the treatment T9 was appeared the effective treatment in reducing seedling mortality (73.34%), disease incidence (84.72%) as well as disease severity (84.21%) caused by the pathogen of R. solani.Moreover, treatment T9 was the best treatment not only for management of the soybean root rot disease but also increased the significant quantity of yield (126.53%).