化学
电化学发光
三元运算
检出限
三元络合物
生物传感器
信号(编程语言)
谷胱甘肽
组合化学
阳极
分子
电极
色谱法
物理化学
有机化学
酶
生物化学
计算机科学
程序设计语言
作者
Rui Zhang,Xia Zhong,Anyi Chen,Jiali Liu,Sheng-Kai Li,Yaqin Chai,Ying Zhuo,Ruo Yuan
标识
DOI:10.1021/acs.analchem.8b05795
摘要
A sensitive electrochemiluminescence (ECL) biosensor was developed for glutathione (GSH) detection based on a novel Ru(bpy)2(cpaphen)2+/TPrA/TiO2 ternary ECL system with Mn2+ as substitute target for signal amplification. Specifically, the TiO2 nanoneedles (TiO2 NNs) were used as the coreaction accelerator for the first time to promote the oxidation process of coreactant tripropylamine (TPrA) in the anode and significantly increase the ECL signal of Ru(bpy)2(cpaphen)2+ for an amplified initial signal. Meanwhile, a novel target conversion strategy for GSH was developed by reducing MnO2 nanosheets to Mn2+ as a substitute target, which played the role of a coenzyme factor for cleaving DNA double strands intercalated with Ru(bpy)2(cpaphen)2+ to markedly weaken initial signal. As a result, the novel “on–off” biosensor achieved a sensitive detection of GSH range from 5 μM to 215 μM with a detection limit of 0.33 μM. Importantly, the proposed strategy enriched the application of Ru complex and TPrA ECL system in bioanalytical applications, and provided a new signal amplification strategy for bioactive small molecules.
科研通智能强力驱动
Strongly Powered by AbleSci AI