线粒体
膜
细胞器
细胞生物学
线粒体内膜
生物
细胞质
细胞膜
生物物理学
膜间隙
作者
Gabriel Arismendi-Morillo
出处
期刊:Methods of Molecular Biology
日期:2021-01-01
卷期号:2277: 449-461
被引量:1
标识
DOI:10.1007/978-1-0716-1270-5_28
摘要
Conventional transmission electron microscopy is an essential tool to understand the structure-function relationships and play a vital role in biological research. Mitochondria-associated membranes are linked with cancer processes in a fundamental manner. A conventional transmission electron microscopy method for preparing specimens in clinical and research settings for the study-analysis of the mitochondria-associated membranes in human tumors is presented. The sample processing includes chemical fixation by immersion, dehydration, embedding, polymerization, sectioning, and staining.
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