An inferior-superior colliculus circuit controls auditory cue-directed visual spatial attention

Selective attention modulates neuronal activity in multiple brain regions, but the origins of attention signals remain unclear. We show that, during a visual task requiring spatial attention directed by an auditory cue, an inferior-superior colliculus circuit provides the key attention signal. In mice performing a task based on a visual stimulus in the cued hemifield while ignoring a conflicting stimulus on the uncued side, the visual cortex (V1) and superior colliculus (SC) showed strong attentional modulation, with a shorter latency in the SC. The nucleus of the brachium of the inferior colliculus (nBIC), which provides auditory inputs to the SC, was activated not only at auditory cue onset but also during the delay period before the visual stimulus. The delay activity, but not cue onset activity, was crucial for task performance and attentional modulation in the SC and V1. These results establish a new behavioral paradigm for studying visual attention in mice and identify a midbrain signal controlling auditory cue-directed spatial attention.