戊二醛
京尼平
静电纺丝
纳米纤维
极限抗拉强度
细胞外基质
材料科学
形态学(生物学)
组织工程
化学
细胞粘附
化学工程
粘附
高分子化学
生物医学工程
聚合物
复合材料
壳聚糖
有机化学
生物化学
工程类
生物
遗传学
医学
作者
Xueshi Luo,Zhenzhao Guo,Ping He,Tian Chen,Lihua Li,Shan Ding,Hong Li
标识
DOI:10.1016/j.ijbiomac.2018.01.179
摘要
Collagen electrospun scaffolds properly reproduce the framework of the extracellular matrix (ECM) of tissues that are natural with the fibrous morphology of the protein by coupling large biomimetism of the biological material. However, traditional solvents employed for collagen electrospinning lead to poor mechanical attributes and bad hydro-stability. In this work, by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride with N-hydroxysulfosuccinimide (EDC-NHS), glutaraldehyde (GTA) and genipin (GP) respectively, electrospun collagen fibers cross-linked, effectively stabilized the fiber morphology over 2months and improved the mechanical properties in both dry and wet state, especially EDC-NHS with large ultimate tensile stress and εb. The secondary structure of collagen structure still remained and had no obvious difference among various crosslinked samples according to FTIR. On the cell assessment, electrospun collagen fibers crosslinked by EDC-NHS, GTA and GP, were found to support cell adhesion, spreading and proliferation of MC3T3-E1. By contrast, GTA was more effective in preserving explicit fibrous morphology with a relatively lower cell viability both in FBS and BSA soaked mats. Interestingly, GP also had the similar cytocompatibility of MC3T3-E1 as EDC-NHS did. The study proved the feasibility of chemical crosslinker to electrospun collagen for biomedical application.
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