多巴胺转运体
酪氨酸羟化酶
前脑
多巴胺能
中脑
多巴胺
生物
绿色荧光蛋白
神经科学
细胞生物学
内科学
内分泌学
中枢神经系统
遗传学
基因
医学
作者
Siew Hoong Yip,Jade York,Brian I. Hyland,Stephen J. Bunn,David R. Grattan
标识
DOI:10.1016/j.jchemneu.2017.12.002
摘要
Co-localization of the expression of the dopamine transporter (DAT) with the catecholamine synthesising enzyme tyrosine hydroxylase (TH) has been investigated using transgenic mice expressing Cre recombinase (Cre) dependent green fluorescent protein (GFP) under the control of the DAT promoter (DATIREScre/GFP). Brain sections from adult female mice were stained for Cre-induced GFP and TH using immunohistochemistry, revealing a high degree of co-expression in the midbrain dopaminergic neurons (A8-10) with the exception of the periaqueductal and dorsal raphe nuclei where dual-labelling was notably lower. In contrast, most of the rostral groups of TH-expressing neurons in the forebrain (A11, A13 - A15) showed little or no co-localization with Cre-induced GFP. Interestingly, a subpopulation of about 30% of the TH-immunoreactive neurons in the arcuate nucleus (A12) also express GFP staining. This observation supports the proposal that this hypothalamic cluster of dopaminergic neurons is neurochemically, and thus potentially functionally, heterogeneous. This study extends earlier literature focusing primarily on DAT expression in midbrain structures to demonstrate a heterogeneity of DAT and TH co-localization in forebrain neurons, particularly those in the hypothalamus. It also highlights the importance of carefully selecting and validating transgenic mouse lines when studying dopaminergic neurons.
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